rabbit anti cyclin b1 Search Results


ccnb1 antibody gtx100911  (GeneTex)
90
GeneTex ccnb1 antibody gtx100911
Knockdwon of <t>cyclin</t> <t>B1</t> inhibits tumorigenicity of chondrosarcoma. A. CH2879 cells were infected with lentivirus expressing control or FOXA1 shRNA. qRT-PCR of <t>CCNB1</t> gene expression in CH2879 with control or FOXA1 shRNA. B. CH2879 cell lines stably expressing vector control or FOXA1 were used in this assay. qRT-PCR of CCNB1 gene expression in CH2879 stable cell lines. C and D. Lysates of CH2879 cells were immunoblotted as indicated antibodies. Actin served as the control. E. MTT assay was performed to assess cell proliferation at different time points including 24, 48, 72 and 96 hr. F. Images and quantification of colony forming ability assay of CH2879 cells. G. CH2879 cell lines stably expressing vector control or FOXA1 were infected with control or two specific cyclin B1 shRNA. Lysates were harvested and immunoblotted. H. MTT assay of indicated cells was determined for cell proliferation at different time points including 24, 48, 72, and 96 hr. Data are means ± SD, Student’s t-test. *P < 0.05, **P < 0.01. ***P < 0.001.
Ccnb1 Antibody Gtx100911, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccnb1 antibody gtx100911/product/GeneTex
Average 90 stars, based on 1 article reviews
ccnb1 antibody gtx100911 - by Bioz Stars, 2026-02
90/100 stars
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anti-cyclin b1 gtx100911  (GeneTex)
90
GeneTex anti-cyclin b1 gtx100911
Knockdwon of <t>cyclin</t> <t>B1</t> inhibits tumorigenicity of chondrosarcoma. A. CH2879 cells were infected with lentivirus expressing control or FOXA1 shRNA. qRT-PCR of <t>CCNB1</t> gene expression in CH2879 with control or FOXA1 shRNA. B. CH2879 cell lines stably expressing vector control or FOXA1 were used in this assay. qRT-PCR of CCNB1 gene expression in CH2879 stable cell lines. C and D. Lysates of CH2879 cells were immunoblotted as indicated antibodies. Actin served as the control. E. MTT assay was performed to assess cell proliferation at different time points including 24, 48, 72 and 96 hr. F. Images and quantification of colony forming ability assay of CH2879 cells. G. CH2879 cell lines stably expressing vector control or FOXA1 were infected with control or two specific cyclin B1 shRNA. Lysates were harvested and immunoblotted. H. MTT assay of indicated cells was determined for cell proliferation at different time points including 24, 48, 72, and 96 hr. Data are means ± SD, Student’s t-test. *P < 0.05, **P < 0.01. ***P < 0.001.
Anti Cyclin B1 Gtx100911, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cyclin b1 gtx100911/product/GeneTex
Average 90 stars, based on 1 article reviews
anti-cyclin b1 gtx100911 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rabbit anti-cyclin b1 (d5c10) antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology rabbit anti-cyclin b1 (d5c10) antibody
Knockdwon of <t>cyclin</t> <t>B1</t> inhibits tumorigenicity of chondrosarcoma. A. CH2879 cells were infected with lentivirus expressing control or FOXA1 shRNA. qRT-PCR of <t>CCNB1</t> gene expression in CH2879 with control or FOXA1 shRNA. B. CH2879 cell lines stably expressing vector control or FOXA1 were used in this assay. qRT-PCR of CCNB1 gene expression in CH2879 stable cell lines. C and D. Lysates of CH2879 cells were immunoblotted as indicated antibodies. Actin served as the control. E. MTT assay was performed to assess cell proliferation at different time points including 24, 48, 72 and 96 hr. F. Images and quantification of colony forming ability assay of CH2879 cells. G. CH2879 cell lines stably expressing vector control or FOXA1 were infected with control or two specific cyclin B1 shRNA. Lysates were harvested and immunoblotted. H. MTT assay of indicated cells was determined for cell proliferation at different time points including 24, 48, 72, and 96 hr. Data are means ± SD, Student’s t-test. *P < 0.05, **P < 0.01. ***P < 0.001.
Rabbit Anti Cyclin B1 (D5c10) Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-cyclin b1 (d5c10) antibody/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
rabbit anti-cyclin b1 (d5c10) antibody - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti-cyclin t1 genetex gtx133413  (GeneTex)
90
GeneTex rabbit anti-cyclin t1 genetex gtx133413
Knockdwon of <t>cyclin</t> <t>B1</t> inhibits tumorigenicity of chondrosarcoma. A. CH2879 cells were infected with lentivirus expressing control or FOXA1 shRNA. qRT-PCR of <t>CCNB1</t> gene expression in CH2879 with control or FOXA1 shRNA. B. CH2879 cell lines stably expressing vector control or FOXA1 were used in this assay. qRT-PCR of CCNB1 gene expression in CH2879 stable cell lines. C and D. Lysates of CH2879 cells were immunoblotted as indicated antibodies. Actin served as the control. E. MTT assay was performed to assess cell proliferation at different time points including 24, 48, 72 and 96 hr. F. Images and quantification of colony forming ability assay of CH2879 cells. G. CH2879 cell lines stably expressing vector control or FOXA1 were infected with control or two specific cyclin B1 shRNA. Lysates were harvested and immunoblotted. H. MTT assay of indicated cells was determined for cell proliferation at different time points including 24, 48, 72, and 96 hr. Data are means ± SD, Student’s t-test. *P < 0.05, **P < 0.01. ***P < 0.001.
Rabbit Anti Cyclin T1 Genetex Gtx133413, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-cyclin t1 genetex gtx133413/product/GeneTex
Average 90 stars, based on 1 article reviews
rabbit anti-cyclin t1 genetex gtx133413 - by Bioz Stars, 2026-02
90/100 stars
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human anti rabbit tnf  (Boster Bio)
90
Boster Bio human anti rabbit tnf
Knockdwon of <t>cyclin</t> <t>B1</t> inhibits tumorigenicity of chondrosarcoma. A. CH2879 cells were infected with lentivirus expressing control or FOXA1 shRNA. qRT-PCR of <t>CCNB1</t> gene expression in CH2879 with control or FOXA1 shRNA. B. CH2879 cell lines stably expressing vector control or FOXA1 were used in this assay. qRT-PCR of CCNB1 gene expression in CH2879 stable cell lines. C and D. Lysates of CH2879 cells were immunoblotted as indicated antibodies. Actin served as the control. E. MTT assay was performed to assess cell proliferation at different time points including 24, 48, 72 and 96 hr. F. Images and quantification of colony forming ability assay of CH2879 cells. G. CH2879 cell lines stably expressing vector control or FOXA1 were infected with control or two specific cyclin B1 shRNA. Lysates were harvested and immunoblotted. H. MTT assay of indicated cells was determined for cell proliferation at different time points including 24, 48, 72, and 96 hr. Data are means ± SD, Student’s t-test. *P < 0.05, **P < 0.01. ***P < 0.001.
Human Anti Rabbit Tnf, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human anti rabbit tnf/product/Boster Bio
Average 90 stars, based on 1 article reviews
human anti rabbit tnf - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Knockdwon of cyclin B1 inhibits tumorigenicity of chondrosarcoma. A. CH2879 cells were infected with lentivirus expressing control or FOXA1 shRNA. qRT-PCR of CCNB1 gene expression in CH2879 with control or FOXA1 shRNA. B. CH2879 cell lines stably expressing vector control or FOXA1 were used in this assay. qRT-PCR of CCNB1 gene expression in CH2879 stable cell lines. C and D. Lysates of CH2879 cells were immunoblotted as indicated antibodies. Actin served as the control. E. MTT assay was performed to assess cell proliferation at different time points including 24, 48, 72 and 96 hr. F. Images and quantification of colony forming ability assay of CH2879 cells. G. CH2879 cell lines stably expressing vector control or FOXA1 were infected with control or two specific cyclin B1 shRNA. Lysates were harvested and immunoblotted. H. MTT assay of indicated cells was determined for cell proliferation at different time points including 24, 48, 72, and 96 hr. Data are means ± SD, Student’s t-test. *P < 0.05, **P < 0.01. ***P < 0.001.

Journal: American Journal of Cancer Research

Article Title: FOXA1 transcriptionally up-regulates cyclin B1 expression to enhance chondrosarcoma progression

doi:

Figure Lengend Snippet: Knockdwon of cyclin B1 inhibits tumorigenicity of chondrosarcoma. A. CH2879 cells were infected with lentivirus expressing control or FOXA1 shRNA. qRT-PCR of CCNB1 gene expression in CH2879 with control or FOXA1 shRNA. B. CH2879 cell lines stably expressing vector control or FOXA1 were used in this assay. qRT-PCR of CCNB1 gene expression in CH2879 stable cell lines. C and D. Lysates of CH2879 cells were immunoblotted as indicated antibodies. Actin served as the control. E. MTT assay was performed to assess cell proliferation at different time points including 24, 48, 72 and 96 hr. F. Images and quantification of colony forming ability assay of CH2879 cells. G. CH2879 cell lines stably expressing vector control or FOXA1 were infected with control or two specific cyclin B1 shRNA. Lysates were harvested and immunoblotted. H. MTT assay of indicated cells was determined for cell proliferation at different time points including 24, 48, 72, and 96 hr. Data are means ± SD, Student’s t-test. *P < 0.05, **P < 0.01. ***P < 0.001.

Article Snippet: Whole-cell lysates were analyzed with the following antibodies: FOXA1 (GeneTex, GTX100308), p53 (Cell Signaling, #2524), p21 (CALBIOCHEM, OP64), CCNB1 (GeneTex, GTX100911), and β-actin (GeneTex, GTX109639).

Techniques: Infection, Expressing, Control, shRNA, Quantitative RT-PCR, Gene Expression, Stable Transfection, Plasmid Preparation, MTT Assay

FOXA1 binds specifically to the CCNB1 promoter in chondrosarcoma cells. A. The FOXA1 binding motif on CCNB1 promoter was predicted by PROMO 3.0 and is visually represented in WebLogo (http://weblogo.berkeley.edu). A schematic of the FOXA1 binding site located in CCNB1 promoter region within -1000 and -400 bp to transcription starting site. B. Luciferase reporter assay of the promoter activities of CCNB1 promoter-deletion mutants with or without ectopic expression of FOXA1 in 293 cells. C. One FOXA1 binding site located in the CCNB1 promoter within -400 bp to transcription starting site was mutated by site direct mutagenesis. Reporter assay of CCNB1 promoter activity with FOXA1-binding site mutations in 293 cells ectopically expressing vector control or FOXA1. D. CH2879 cells stably expressing vector control or FOXA1 were subjected to ChIP assay. Cross-linked chromatin was immunoprecipitated by utilizing FOXA1 or IgG antibodies. The input and immunoprecipitated DNA were subjected to q-PCR primers corresponding to the promoter region of CCNB1 (Table S1). P values were determined by Student’s t-test, error bars represent ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: American Journal of Cancer Research

Article Title: FOXA1 transcriptionally up-regulates cyclin B1 expression to enhance chondrosarcoma progression

doi:

Figure Lengend Snippet: FOXA1 binds specifically to the CCNB1 promoter in chondrosarcoma cells. A. The FOXA1 binding motif on CCNB1 promoter was predicted by PROMO 3.0 and is visually represented in WebLogo (http://weblogo.berkeley.edu). A schematic of the FOXA1 binding site located in CCNB1 promoter region within -1000 and -400 bp to transcription starting site. B. Luciferase reporter assay of the promoter activities of CCNB1 promoter-deletion mutants with or without ectopic expression of FOXA1 in 293 cells. C. One FOXA1 binding site located in the CCNB1 promoter within -400 bp to transcription starting site was mutated by site direct mutagenesis. Reporter assay of CCNB1 promoter activity with FOXA1-binding site mutations in 293 cells ectopically expressing vector control or FOXA1. D. CH2879 cells stably expressing vector control or FOXA1 were subjected to ChIP assay. Cross-linked chromatin was immunoprecipitated by utilizing FOXA1 or IgG antibodies. The input and immunoprecipitated DNA were subjected to q-PCR primers corresponding to the promoter region of CCNB1 (Table S1). P values were determined by Student’s t-test, error bars represent ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Whole-cell lysates were analyzed with the following antibodies: FOXA1 (GeneTex, GTX100308), p53 (Cell Signaling, #2524), p21 (CALBIOCHEM, OP64), CCNB1 (GeneTex, GTX100911), and β-actin (GeneTex, GTX109639).

Techniques: Binding Assay, Luciferase, Reporter Assay, Expressing, Mutagenesis, Activity Assay, Plasmid Preparation, Control, Stable Transfection, Immunoprecipitation

Knockdown of FOXA1 attenuates chondrosarcoma tumor growth in vivo. A. Representative images of tumors harvested from subcutaneous mice xenografted with CH2879-control or FOXA1-knockdown cells. B. Tumor volume was monitored every 3 days and plotted. n = 4 per group. C. A proposed model of FOXA1 transcriptionally mediated chondrosarcoma cell cycle progress. In chondrosarcoma cells, FOXA1 binds specifically to the indicated region of CCNB1 promoter to facilitate its expression and thereby promote G2/M transition via cycllinB1-CDK1 complex activation in the cell cycle. Data are shown as means ± SD; Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: American Journal of Cancer Research

Article Title: FOXA1 transcriptionally up-regulates cyclin B1 expression to enhance chondrosarcoma progression

doi:

Figure Lengend Snippet: Knockdown of FOXA1 attenuates chondrosarcoma tumor growth in vivo. A. Representative images of tumors harvested from subcutaneous mice xenografted with CH2879-control or FOXA1-knockdown cells. B. Tumor volume was monitored every 3 days and plotted. n = 4 per group. C. A proposed model of FOXA1 transcriptionally mediated chondrosarcoma cell cycle progress. In chondrosarcoma cells, FOXA1 binds specifically to the indicated region of CCNB1 promoter to facilitate its expression and thereby promote G2/M transition via cycllinB1-CDK1 complex activation in the cell cycle. Data are shown as means ± SD; Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Whole-cell lysates were analyzed with the following antibodies: FOXA1 (GeneTex, GTX100308), p53 (Cell Signaling, #2524), p21 (CALBIOCHEM, OP64), CCNB1 (GeneTex, GTX100911), and β-actin (GeneTex, GTX109639).

Techniques: Knockdown, In Vivo, Control, Expressing, Activation Assay